視網(wǎng)膜是位于眼睛后部的多層結(jié)構(gòu)。它包括感光細(xì)胞和視網(wǎng)膜色素上皮細(xì)胞（RPEpiC）。RPEpiC位于神經(jīng)視網(wǎng)膜和脈絡(luò)膜之間，形成視網(wǎng)膜外屏障，控制著視網(wǎng)膜下腔的化學(xué)構(gòu)成。人們發(fā)現(xiàn)RPEpiC內(nèi)有許多特定偏振分布的細(xì)胞內(nèi)蛋白質(zhì)。與其他上皮細(xì)胞通常頂生面沒有基質(zhì)相比，RPEpiC的頂生面胞膜是與細(xì)胞外基質(zhì)相接觸的。RPEpiC在調(diào)節(jié)相鄰的感光膜周轉(zhuǎn)、維生素A的代謝、保護(hù)細(xì)胞內(nèi)黑色素所致的視網(wǎng)膜光損害中起關(guān)鍵作用。RPEpiC與很多視網(wǎng)膜疾病的發(fā)病相關(guān)，如增殖性視網(wǎng)膜病變和年齡相關(guān)性黃斑變性。
Sciencell研究實(shí)驗(yàn)室的HRPEpiC是從人視網(wǎng)膜分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度超過5×10^5/ml。細(xì)胞特性經(jīng)cytokeratine -18, -19和纖維粘連蛋白免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞10倍增增殖。
推薦培養(yǎng)基：上皮細(xì)胞培養(yǎng)基 (EpiCM, Cat. #4101)
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貨號(hào)
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6540
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產(chǎn)地
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美國
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縮寫
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HRPEpiC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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關(guān)鍵字
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人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC 人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC 人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC 人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC 人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC 人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC 人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC 人視網(wǎng)膜色素上皮細(xì)胞 HRPEpiC
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公司簡(jiǎn)介
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合肥星肽生物科技有限公司作為專業(yè)的ScienCell中國代理商，專業(yè)經(jīng)營實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基、原代細(xì)胞無血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無血清培養(yǎng)基的研究和開發(fā)。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù)，對(duì)購買項(xiàng)目的前期資料提供,中期合同保證,后期貨物跟蹤到 *終售后的確保項(xiàng)目準(zhǔn)確到位，都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務(wù)！
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聯(lián)系方式
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何經(jīng)理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898，
E-mail：info@qingbio.com http://th47.cn QQ:514713116
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1.) Favret S, Binet F, Lapalme E, Leboeuf D, Carbadillo J, Rubic T, Picard E, Mawambo G, Tetreault N, Jean- Joyal S, Chemtob S, Sennlaub F, SanGiovanni JP, Guimond M,Sapieha P. (2013) "Deficiency in the metabolite receptor SUCNR1 (GPR91) leads to outer retinal lesions." Aging (Albany NY). 5: 427.
2.) Miyamoto N, Izumi H, Miyamoto R, Bin H, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Transcriptional Regulation of Activating Transcription Factor 4 under Oxidative Stress in Retinal Pigment Epithelial ARPE-19/HPV-16 Cells." Invest Ophthalmol Vis Sci. 52: 1226-34.
3.) Narayan S, Prasanna G, Tchedre K, Krishnamoorthy R, Yorio T. (2010) "Thrombin-induced endothelin-1 synthesis and secretion in retinal pigment epithelial cells is rho kinase dependent." J Ocul Pharmacol Ther. 26: 389-97.
4.) Bertram KM, Baglole CJ, Phipps RP, Libby RT. (2009) "Molecular regulation of cigarette smoke induced-oxidative stress in human retinal pigment epithelial cells: implications for age-related macular degeneration." Am J Physiol Cell Physiol. 297: C1200-10.
5.) Mannermaa E, Vellonen KS, Ryh?nen T, Kokkonen K, Ranta VP, Kaarniranta K, Urtti A. (2009) "Efflux protein expression in human retinal pigment epithelium cell lines."Pharm Res. 26: 1785-91.
6.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
7.) Swaney JS, Moreno KM, Gentile AM, Sabbadini RA, Stoller GL. (2008) "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res. 87: 367-75.
8.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
Question:
Regarding Human Retinal Pigment Epithelial Cells Catalog #6540 Are the cells from a single human donor or multiple in a pool? If single donor, can one order vials from a few donors to compare? Can you provide a list of available donors and their characteristics? I have read the following paper where this product is used and they make the comment that they conducted their work with hRPEpC in multiple donors. Toxicol Sci. 2015 Mar;144(1):163-72. Retinal Toxicity, in vivo and in vitro, Associated with Inhibition of Nicotinamide Phosphoribosyltransferase.
Answers:
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Hi Elizabeth,
it is Single donor.if you are interested in multiple vials from different lots to get different donors. Please contact us at techsupport@sciencellonline.com to obtain donor information.
Thanks for your interest in our products.
SCIENCELL TEAM
